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Eosin Methylene Blue Agar are dyes that combine to form a complex at an acid pH. Small amounts of this dye effectively inhibit the growth of most gram-positive bacteria. Eosin is a dye that responds to changes in pH, going from colorless to black under acidic conditions. At a sufficiently low pH, strong lactose fermenters such as Escherichia coli produce colonies with a green metallic sheen. This metallic green sheen is an indicator of vigorous lactose fermentation ability typical of fecal coliforms. A smaller amount of acid production, which is a result of slow fermentation (by slow lactose-fermenting organisms), gives a brown-pink coloration of growth. Colonies of non-lactose fermenters appear as translucent or pink. Agar is the solidifying agent.
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